ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (1): 198-206.doi: 10.11843/j.issn.0366-6964.2016.01.027

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Study on the Plasma Metabolism Using 1H-NMR in Postpartum Cows

TAO Jin-zhong*,GUO Yan-sheng   

  1. (College of Agriculture,Ningxia University,Yinchuan 750021,China)
  • Received:2015-06-20 Online:2016-01-23 Published:2016-01-23

Abstract:

In postpartum,cows often suffer from negative energy balance,resulting in physical dysfunction.The aim of this study was to define the physiological mechanism of cows exercising metabolic energy shortage after calving.1H-NMR technology was used for measurement of metabolites.Results were analyzed in multivariate statistical methods to find out the trend of plasma metabolites changing after calving and the influence on energy metabolism pathway.Results showed that:the biomarkers profile of plasma was not significantly different in Day 7 from Day 1 after calving;but a significant difference were found between Day 14 and Day 1 after calving.The biomarkers are unsaturated fatty acid,choline and trimethylamine oxide;There were significant differences in biomarkers profile between Day 28 and Day 1 after calving with different markers of unsaturated fatty acid,choline,trimethylamine oxide,citric acid and glucose.After calving,unsaturated fatty acid,choline and tricarboxylic acid expression gradually increased,whereas trimethylamine oxide and glucose expression gradually decreased.With the increasing lactating yield after calving,cows showed a negative energy balance.In response,the lipolysis and fatty acid oxidation,the oxidation of choline,re-methylation methionine and methyl metabolic pathways were increased,relieving the negative energy balance.This study could provide a scientific basis for the physiological mechanism of the negative energy balance after calving for dairy cows.

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